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Question

In genetic engineering antibiotics are used

A
as selectable markers
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B
to select healthy vectors
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C
as sequences from where replication starts
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D
to keep the cultures free of infection.
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Solution

The correct option is A as selectable markers
Selectable markers are those genes that help in selecting those host cells which contain vectors (i.e., transformants) and eliminating the non-transformants. The genes encoding resistance to antibiotics such as tetracycline, ampicillin, kanamycin etc., are useful selectable markers for E.coli. Plasmid pBR322 has two resistance genes – ampicillin resistance (ampr) and tetracycline resistance (tetr) which are considered useful for selectable markers.

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