Question

In initial stages of protoplast culture, sorbitol or mannitol is added as

• A. Source of carbon
• B. Osmotic stabilizer
• C. Additional source of energy
• D. To keep cells alive after removal of cell wall

Answer 1

The correct option is B Osmotic stabilizer

To keep cell alive after the removal of cell wall, sorbitol or mannitol is added as a osmotic stabilizer in the initial stages of protoplast culture.

So, option B is the correct option.