In initial stages of protoplast culture, sorbitol or mannitol is added as
A
Source of carbon
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B
Osmotic stabilizer
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C
Additional source of energy
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D
To keep cells alive after removal of cell wall
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Solution
The correct option is B Osmotic stabilizer To keep cell alive after the removal of cell wall, sorbitol or mannitol is added as a osmotic stabilizer in the initial stages of protoplast culture.