In leaves of C4 plants, malic acid synthesis during carbon dioxide fixation occurs in
In C4 plants, the Hatch and Slack pathway involves two carboxylation reaction, one taking place in chloroplast of mesophyll cells and other in chloroplast of bundle – sheath cells. In mesophyll cells, phosphoenol pyruvate (PEP) is carboxylated to oxaloacetate (OAA) which is then reduced to malic acid.