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Question

In Lederberg's replica plating experiment, what should be used to obtain streptomycin resistant strain?

A
Minimal medium and streptomycine
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B
Complete medium and streptomycine
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C
Only minimal medium
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D
Only complete medium
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Solution

The correct option is B Complete medium and streptomycine
Some bacteria from their population are able to grow in the streptomycin containing medium because they carry the streptomycin-resistant gene. These strains get selected in streptomycin containing the medium. Lederberg, by performing replica plating experiment containing complete medium with antibiotic (streptomycin), demonstrated the process of selection of streptomycin-resistant strains.
So, the correct answer is 'Complete medium and streptomycin'.

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