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Question

In order to estimate the gross primary productivity (GPP) of a pond containing phytoplankton and zooplankton, a student collected water samples from the pond in two bottles labelled X and Y. The dissolved oxygen (DO) of the samples immediately after collection was 8mg ml−1. The DO content when measured after 24 hours of incubation was as follows:
Bottle X: 2 mg ml−1 after incubation in dark
Bottle Y: 18 mg ml−1 after incubation in light
GPP of the pond is?

A
6
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B
10
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C
16
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D
20
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Solution

The correct option is B 16
GPP or Gross Primary productivity is referred as the total rate of photosynthesis including the organic matter used up in respiration during the measurement period.
When the bottles X and Y containing pond water are kept in dark and light respectively, there is decline in Dissolved oxygen (DO) in bottle X because phytoplankton and zooplankton will not be able to photosynthesise and the oxygen will be used up in respiration whereas, there is increase in DO in bottle Y because phytoplankton and zooplankton will photosynthesise in presence of light resulting in production of oxygen.
So, from the information given,
Respiratory activity can be calculated as : Initial DO - DO after incubation in dark = 8 - 2= 6 mg/ml
Net photosynthetic activity can be calculated as : DO after incubation in light - Initial DO = 18 - 8= 10 mg/ml
GPP = Net photosynthetic activity + Respiratory activity = 10 + 6 = 16.
So, the correct answer is C.

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