Question

In order to obtain virus-free plants through tissue culture, the best method is

• A. Anther culture
• B. Meristem culture
• C. Protoplast culture
• D. Embryo rescue

Answer 1

The correct option is B Meristem culture

It is possible to produce disease-free plants through meristem culture because viruses move readily in the vascular system, which, in meristems, is absent. A high metabolic activity in the actively dividing meristematic cells does not allow virus replication and a high endogenous auxin level in shoot apices may inhibit virus multiplication

So, the correct answer is 'Meristem culture'