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Question

In order to obtain virus-free plants through tissue culture, the best method is

A
Anther culture
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B
Meristem culture
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C
Protoplast culture
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D
Embryo rescue
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Solution

The correct option is B Meristem culture
It is possible to produce disease-free plants through meristem culture because viruses move readily in the vascular system, which, in meristems, is absent. A high metabolic activity in the actively dividing meristematic cells does not allow virus replication and a high endogenous auxin level in shoot apices may inhibit virus multiplication
So, the correct answer is 'Meristem culture'

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