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Question
In order to select for cells that successfully integrated the DNA, the type of nutrient media that should be added includes
In order to select for cells that successfully integrated the DNA, the type of nutrient media that should be added includes
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Solution
The correct option is C Plates with no added tryptophan but with streptomycin
Since the recombinant cell has gene for tryptophan synthesis, nutrient media should not contain tryptophan and the bacterial colony should be allowed to synthesize it. The recombinant bacteria have a gene for streptomycin resistance (serving as a selectable marker here), these cells would be able to survive on a nutrient media that have no tryptophan but streptomycin. The nonrecombinant cells would not be able to survive in absence of tryptophan as well as in presence of streptomycin. Thus, the correct answer is option C.
Since the recombinant cell has gene for tryptophan synthesis, nutrient media should not contain tryptophan and the bacterial colony should be allowed to synthesize it. The recombinant bacteria have a gene for streptomycin resistance (serving as a selectable marker here), these cells would be able to survive on a nutrient media that have no tryptophan but streptomycin. The nonrecombinant cells would not be able to survive in absence of tryptophan as well as in presence of streptomycin. Thus, the correct answer is option C.
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