1
Question
In order to test the peptide bond in decapeptide, how many of the following tests can be carried out:
1. Ninhydrin test
2. Biuret test
3. Xanthoproteic test
4. Ceric ammonium test
5. Carbylamine test
1. Ninhydrin test
2. Biuret test
3. Xanthoproteic test
4. Ceric ammonium test
5. Carbylamine test
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Solution
The ninhydrin test is a chemical test which is used to check whether a given analyte contains amines or α-amino acids.
The biuret test is a chemical test that can be used to check for the presence of peptide bonds in a given analyte. Therefore, the biuret test can be also be used to gauge the amount of protein present in the analyte. In this test, the presence of peptides results in the formation of pale purple coloured coordination compounds of the copper(II) ion (when the solution is sufficiently alkaline).
Xanthoproteic test is a biochemical test for the detection of amino acids containing phenolic or indolic groups like phenylalanine, tyrosine, and tryptophan (aromatic amino acids). The test is named Xanthoproteic test due to the formation of a yellow precipitate of xanthoproteic acid.
Alcoholic compounds on reaction with ceric ammonium nitrate
give a red colouration due to the formation of a complex
The chemical reaction is given below.
(NH4)2[Ce(NO3)6]+3ROH→[Ce(NO3)4(ROH)3]+2NH4NO3
(NH4)2[Ce(NO3)6]+3CH3OH→[Ce(NO3)4(CH3OH)3]+2NH4NO3
This is Carbylamine reaction which is used to detect primary amines. The product formed is isocyanide which has foul smelling and can be easily detected. Secondary and tertiary amines dont give this reaction.
The reaction is as follows:
RNH2 + CHCl3 + 3KOH→RNC + 3KCl + +3H2O
Hence answer is 1.
The biuret test is a chemical test that can be used to check for the presence of peptide bonds in a given analyte. Therefore, the biuret test can be also be used to gauge the amount of protein present in the analyte. In this test, the presence of peptides results in the formation of pale purple coloured coordination compounds of the copper(II) ion (when the solution is sufficiently alkaline).
Xanthoproteic test is a biochemical test for the detection of amino acids containing phenolic or indolic groups like phenylalanine, tyrosine, and tryptophan (aromatic amino acids). The test is named Xanthoproteic test due to the formation of a yellow precipitate of xanthoproteic acid.
Alcoholic compounds on reaction with ceric ammonium nitrate
give a red colouration due to the formation of a complex
The chemical reaction is given below.
(NH4)2[Ce(NO3)6]+3ROH→[Ce(NO3)4(ROH)3]+2NH4NO3
(NH4)2[Ce(NO3)6]+3CH3OH→[Ce(NO3)4(CH3OH)3]+2NH4NO3
This is Carbylamine reaction which is used to detect primary amines. The product formed is isocyanide which has foul smelling and can be easily detected. Secondary and tertiary amines dont give this reaction.
The reaction is as follows:
RNH2 + CHCl3 + 3KOH→RNC + 3KCl + +3H2O
Hence answer is 1.
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