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Selection Markers DNA

In pBR322 a...

Question

In $p B R 322 a m p^{R}$ and $t e t^{R}$ genes are present.When we ligate a foreign DNA at $P v u I$ site, then recombiant plasmids will lose the resistance to the ____________.

A

Ampicillin

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B

Tetracycline

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C

Both A & B

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D

None of these

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Solution

The correct option is A Ampicillin
Answer is option A i.e. "Ampicillin"
pBR322 plasmid is a commonly used plasmid cloning vector in E. coli bacteria. Since PvuI restriction site is present in ampicillin resistant gene, when we ligate a foreign DNA at PvuI site, the ampicillin resistant gene gets destroyed. The recombinant plasmid thus formed will lose the resistance to the ampicillin.

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