Question

In $pBR322am{p}^R$ and $te{t}^R$ genes are present.When we ligate a foreign DNA at $PvuI$ site, then recombiant plasmids will lose the resistance to the ____________.

• A. Ampicillin
• B. Tetracycline
• C. Both A & B
• D. None of these

Answer 1

The correct option is A Ampicillin

Answer is option A i.e. "Ampicillin"
pBR322 plasmid is a commonly used plasmid cloning vector in E. coli bacteria. Since PvuI restriction site is present in ampicillin resistant gene, when we ligate a foreign DNA at PvuI site, the ampicillin resistant gene gets destroyed. The recombinant plasmid thus formed will lose the resistance to the ampicillin.