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Gene Silencing

In pest resis...

Question

In pest resistant plants that use gene silencing method, host plant generated dsRNA triggers protection against

A

Rhizobium infestation

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B

Nematode infestation

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C

Bacterial infestation

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D

Nostoc infestation

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Solution

The correct option is B Nematode infestation
Host plant generates dsRNA that triggers protection against nematode infestation. The parasites do not survive in the transgenic host that is capable of expressing specific interfering RNA. This form of gene silencing although highly effective is a little more expensive than the creation of Bt cotton using the bacterium Bacillus thuringiensis.

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Q. Which of the following statements are correct regarding the process of RNA interference?
(i) This is used to prevent the infestation of protozoans.
(ii) It takes place in some eukaryotic and all prokaryotic organisms as a method of cellular defense.
(iii) The method involves silencing of a specific mRNA due to a complementary dsRNA molecule.
(iv) It is a novel strategy to produce pest-resistant plants.

Q. Assertion :The term 'cross-protection' is used specifically for the protection of a plant by preinfecting the host with a mild strain of the pathogens. Reason: Breakdown of resistant genes in plants is responsible for the occurrence of disease in a previously resistant variety of a crop.

Q. Identify the correct sequence of steps in RNA-interference (RNAi) process to develop nematode resistant plants.
i) Silencing of specific mRNA of the nematode
ii) Formation of sense and antisense RNA
iii) dsRNA formation
iv) Introducing nematode-specific genes into plant

Q. Silencing of mRNA has been used in producing transgenic plants resistant to

Q. Silencing of mRNA has been used in producing transgenic plants resistant to _____________.

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