Question

In recombinant DNA technology, a plasmid vector is cleaved by

• A. Modified DNA ligase
• B. A heated allkaline solution
• C. The same enzyme that cleaves the donor DNA
• D. The different enzyme than that cleaves the donor DNA

Answer 1

The correct option is C The same enzyme that cleaves the donor DNA

Answer is option C i.e. "The same enzyme that cleaves the donor DNA"
In rDNA technology, the plasmid vector must be cleaved by specific restriction enzyme. Plasmid vectors are cleaved by same enzyme that cleaves the donor DNA leading to creation of sticky ends which further helps in recombination.