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Question
In recombination DNA technique, which of the following technique is not used in introducing DNA into host cell?
In recombination DNA technique, which of the following technique is not used in introducing DNA into host cell?
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Solution
The correct option is B Replication
- Transformation is the transfer of genetic material from one bacterial strain to another without establishing physical contact.
- Transduction is the transfer of genetic material from one strain to other via viral particles. The virus infects bacteria and released viral particles carry part of the bacterial genetic material which is then transferred to the next host bacterium.
- Electroporation is the method of direct gene transfer by applying a pulse of high voltage to protoplasts/cells/ tissues to make transient pores in the plasma membrane which serve to uptake the foreign DNA.
- Conjugation is the transfer of genetic material between two bacterial strains via the conjugation tube.
- DNA replication is semiconservative in which a DNA strand serves as a template for the synthesis of a new strand and produces two new DNA molecules, each with one new strand and one parental strand; it is not a method of gene transfer.
Thus, the correct answer is option B.
- Transformation is the transfer of genetic material from one bacterial strain to another without establishing physical contact.
- Transduction is the transfer of genetic material from one strain to other via viral particles. The virus infects bacteria and released viral particles carry part of the bacterial genetic material which is then transferred to the next host bacterium.
- Electroporation is the method of direct gene transfer by applying a pulse of high voltage to protoplasts/cells/ tissues to make transient pores in the plasma membrane which serve to uptake the foreign DNA.
- Conjugation is the transfer of genetic material between two bacterial strains via the conjugation tube.
- DNA replication is semiconservative in which a DNA strand serves as a template for the synthesis of a new strand and produces two new DNA molecules, each with one new strand and one parental strand; it is not a method of gene transfer.
Thus, the correct answer is option B.
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