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Question
In recombination process, which vector is used ?
In recombination process, which vector is used ?
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Solution
The correct option is B Agrobacterium tumefaciens
Agrobacterium tumefaciens is a bacterial plant pathogen found in the soil which causes crown gall disease in plants. The basic mechanism that works behind it is, transfer of a segment or small piece of bacterial DNA to the plant genome. This bacterial DNA segment gets incorporated into the plant genome, stabilizes there and transcribes or expresses itself. The bacterial DNA causing disease is situated on a plasmid, Ti plasmid (Tumour inducing plasmid, 200kb) instead of on a bacterial chromosome. Thus, it is used as vector in recombination process.
Agrobacterium tumefaciens is a bacterial plant pathogen found in the soil which causes crown gall disease in plants. The basic mechanism that works behind it is, transfer of a segment or small piece of bacterial DNA to the plant genome. This bacterial DNA segment gets incorporated into the plant genome, stabilizes there and transcribes or expresses itself. The bacterial DNA causing disease is situated on a plasmid, Ti plasmid (Tumour inducing plasmid, 200kb) instead of on a bacterial chromosome. Thus, it is used as vector in recombination process.
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