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Question

In the agrobacterium mediated gene transfer using the leaf disc method, what is the significance of cutting the leaf into discs?


Your Answer
A
To make the leaf round shape
Correct Answer
B
To induce induce dedifferentiation in leaf cells
Your Answer
C
To increase surface area for transfers
Your Answer
D
To use both surfaces of the leaf

Solution

The correct option is C To induce induce dedifferentiation in leaf cells
In leaf disc method for transformation, small discs are cut from a leaf. This induces stress. Stressed leaf disc cells undergo dedifferentiation. Dedifferentiation makes the cells totipotent and with appropriate hormones they can grow into any other plant tissue.

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