Question

In the agrobacterium mediated gene transfer using the leaf disc method, what is the significance of cutting the leaf into discs?

• A. To make the leaf round shape
• B. To induce induce dedifferentiation in leaf cells
• C. To increase surface area for transfers
• D. To use both surfaces of the leaf

Answer 1

The correct option is B To induce induce dedifferentiation in leaf cells

In leaf disc method for transformation, small discs are cut from a leaf. This induces stress. Stressed leaf disc cells undergo dedifferentiation. Dedifferentiation makes the cells totipotent and with appropriate hormones they can grow into any other plant tissue.