Question

In the biochemical characterisation of the transforming principle, it was found that the process of transformation is not affected by which of the following enzymes?
A. DNase
B. RNase
C. Peptidase
D. Lipase

• A. A, B, D
• B. A,C, D
• C. B, C, D
• D. A, B, C

Answer 1

The correct option is C B, C, D

The process of transformation is not affected by RNase, peptidase and lipase.
Transformation is the genetic alteration of a cell that happens as a result of direct uptake and incorporation of genetic material from the surroundings through the cell membrane.
Avery, MacLeod and McCarty used three experimental tubes to which RNases, proteinases and DNase were added along with heat-killed S-strain bacteria and live R-strain bacteria.
Among the three sets of experimental tubes, those to which RNAse and proteinase were added, still retained the ability to transform R-strain to live S-strain. Therefore, neither RNA nor protein could be the transforming principle. It was only in the tubes to which DNase was added, that transformation could not happen. It was thus concluded that DNA is the genetic material.