The correct option is D To use up the starch that was produced earlier.
In the experiment to prove light is necessary for photosynthesis, the plant is kept in dark for three to four days in order to use up the starch that was produced earlier. It is done to avoid false positive results. Starch is produced only when photosynthesis takes place. So when the plant is again exposed to light, photosynthesis will begin and its end product i.e. starch can be tested using iodine test.