(a) Initially, the plant is kept in dark for 24 hours to destarch the leafs.
(b) A part of destarched leaf is covered with black paper so that the light does not reach that region and photosynthesis does not occur.
(c) The chlorophyll is removed from the leaf green leaf before testing by boiling it in alcohol.
(d) The reagent used to test the presence of starch is called iodine.