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Carbon Dioxide Is Given out during Breathing

In the experi...

Question

In the experimental set up on ' $C O_{2}$ is released during respiration,' if one forgets to keep the vial with KOH in the conical flask, how will the result vary? Give details.

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Solution

Respiration in plants can be studied in moist germinating seeds that release carbon dioxide (CO2) during respiration. Potassium hydroxide absorbs carbon dioxide released by the seeds and a partial vacuum is created in the flask as a result. This causes the water level in the delivery tube to rise. As KOH breaks down, the oxygen needed for cellular respiration is released. It serves as a temporary energy source for the respiring organism. It binds with carbon dioxide to form a solid, preventing CO₂ production from affecting gas volume. Its attraction for water will cause water to enter the respirometer.

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Q. In the experiment to show that

C O_{2}

is released during respiration', the small test tube of KOH solution is suspended inside the conical flask to absorb the :
(a) air of the flask
(b) moisture of the flask
(c)

O_{2}

of the flask
(d)

C O_{2}

of the flask released by the seeds

Q. For experiment showing that

C O_{2}

is evolved during the process of respiration in plants - arrange the steps sequentially.

i) Soak gram seeds in water, overnight. Keep them in conical flask.
ii) Close the conical flask with cork fitted with 'U' tube.
iii) Insert test tube with KOH solution in the conical flask.
iv) Keep the set steady for some time.

Q. How does the use of KOH help to show

{C O}_{2}

is released during respiration in the germinating seed respiration experiment ?

Q. A student sets up the apparatus for the experiment to show that

C O_{2}

is released during
respiration. After two hours he would observe :

Q. A student while experimenting on carbon dioxide is given out during respiration, he forgot to take two precautions as given below :

a) To hang a test tube containing KOH solution in the conical flask.
b) To put moist seeds in the conical flask.

State the result of the experiment due to the mistakes caused by him.

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Carbon Dioxide Is Given out during Breathing

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