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Question
In the figure given below, the line (upper) indicates the action spectrum for photosynthesis and the line (lower) indicates the absorption spectrum of chlorophyll
a. Answer the following: What does the action spectrum indicate? How can we plot an action spectrum?
b. How can we derive an absorption spectrum for any substance?
a. Answer the following: What does the action spectrum indicate? How can we plot an action spectrum?
b. How can we derive an absorption spectrum for any substance?
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Solution
(A) Action spectrum :
Action spectrum indicates the maximum rate of photosynthesis by a photosynthetic pigment by measuring the amount of oxygen gas evolved at different wavelengths of light.
Plotting the action spectra :
Action spectra can be plotted by measuring the amount of oxygen released during photosynthesis by a plant at different wavelengths.
(B)Absorption spectra :
Absorption spectra indicate the wavelength of light which is maximum absorbed by any substance.
Deriving absorption spectra :
● Absorption spectra of any substance can be derived by measuring the absorbance of a substance at different wavelengths, such as in the range of 400 nm to 700 nm using a spectrophotometer.
● Then plot a graph of wavelength of light vs absorbance at each wavelength.
●This will give an absorption spectrum of any substance.
Action spectrum indicates the maximum rate of photosynthesis by a photosynthetic pigment by measuring the amount of oxygen gas evolved at different wavelengths of light.
Plotting the action spectra :
Action spectra can be plotted by measuring the amount of oxygen released during photosynthesis by a plant at different wavelengths.
(B)Absorption spectra :
Absorption spectra indicate the wavelength of light which is maximum absorbed by any substance.
Deriving absorption spectra :
● Absorption spectra of any substance can be derived by measuring the absorbance of a substance at different wavelengths, such as in the range of 400 nm to 700 nm using a spectrophotometer.
● Then plot a graph of wavelength of light vs absorbance at each wavelength.
●This will give an absorption spectrum of any substance.
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