Question

In the initial stage of protoplast culture sorbitol/ mannitol is included.

• A. As an additional source of carbon
• B. As an additional source of energy
• C. To keep cells alive after the removal of cell wall
• D. As osmotic stabilizer

Answer 1

The correct option is D As osmotic stabilizer

• Protoplasts of the plants which are need to fusion are isolated and the isolated protoplasts are being fusion and hybrid protoplast is formed which will grow under in vitro conditions and new plants are produced.
• Sorbitol and mannitol which are the sugar alcohols are added during protoplast culture. These are not metabolized by plant cells and this helps to prevent the released protoplast from bursting. So, sorbitol and mannitol are used as osmotic stabilizers to prevent from bursting.

So, the correct option is option D.