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Question
In the preparation of culture media for tissue culture, __________ is used as a solidifying agent.
In the preparation of culture media for tissue culture, __________ is used as a solidifying agent.
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Solution
The correct option is B agar agar
Tissue culture is a technique used in modern plant breeding techniques for the faster vegetative multiplication (micropropagation) of plants. It helps in growing plant cells, tissues or organs (explant) in a sterile culture medium under controlled environmental conditions.
The culture media is prepared by adding appropriate concentrations of below mentioned nutrients:
- Inorganic salts - Helps in providing necessary micro (zinc, manganes, iron, copper, molybdenum, chlorine, boron) and macronutrients (nitrogen, phosphorus, pottassium, calcium, sulphur, magnesium) to the growing cells.
- Carbon source - Sucrose, glucose, fructose, etc for energy and growth.
- Amino acids - Act as nitrogen source for building up proteins.
- Vitamins and growth hormones - Auxins, gibberellins and cytokinins are added to induce growth, development and morphological changes in the explant. Vitamins are essential for proper growth.
- Agar agar - It acts as a solidifying agent in the culture media. That means it helps to convert the liquid medium (broth) to a gel form. Hence option b is the answer.
Tissue culture is a technique used in modern plant breeding techniques for the faster vegetative multiplication (micropropagation) of plants. It helps in growing plant cells, tissues or organs (explant) in a sterile culture medium under controlled environmental conditions.
The culture media is prepared by adding appropriate concentrations of below mentioned nutrients:
- Inorganic salts - Helps in providing necessary micro (zinc, manganes, iron, copper, molybdenum, chlorine, boron) and macronutrients (nitrogen, phosphorus, pottassium, calcium, sulphur, magnesium) to the growing cells.
- Carbon source - Sucrose, glucose, fructose, etc for energy and growth.
- Amino acids - Act as nitrogen source for building up proteins.
- Vitamins and growth hormones - Auxins, gibberellins and cytokinins are added to induce growth, development and morphological changes in the explant. Vitamins are essential for proper growth.
- Agar agar - It acts as a solidifying agent in the culture media. That means it helps to convert the liquid medium (broth) to a gel form. Hence option b is the answer.
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