Question

In tissue culture, callus can be induced to form shoot or root by altering the ratio of

• A. Auxin to cytokinin
• B. Cytokinin to ethylene
• C. Auxin to gibberellin
• D. Gibberellin to cytokinin

Answer 1

Answer: (A)

Auxin to cytokinin

Callus is unorganized actively dividing the mass of cells maintained in culture. It has the meristematic cell which has ample amount of cytoplasm to trigger cell division. It is treated with the growth hormones to allow the cell division and differentiation. Callus is transferred to the media with the high concentration of cytokinin and low concentration of auxin for development of shoot. After the development of shoots, it is transferred to rooting media which contains the high concentration of auxin and low concentration of cytokinin for rooting.

So, the correct answer is option A.