Question

In tissue culture, differentiation of shoot is controlled by ...........................

• A. Light Intensity
• B. Temperature shock
• C. Low Auxin and high CK $\left(High\frac{Cyto}{Auxin}Ratio\right)$
• D. None of the above

Answer 1

The correct option is C Low Auxin and high CK $\left(High\frac{Cyto}{Auxin}Ratio\right)$

Cytokinins promote cell division and differentiation of young, relatively unspecialized cells into mature, more specialized cells in intact plants. They are a required ingredient in any plant tissue culture medium and must be present for cells to divide. In tissue culture, cytokinins interact with auxin during the formation of plant organs such as roots and stems. For example, in tobacco tissue culture a high ratio of auxin to cytokinin induces root formation, whereas a low ratio of auxin to cytokinin induces shoot formation.