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In tissue culture medium, the source of phytohormones is
In tissue culture medium, the source of phytohormones is
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Solution
The correct option is C Coconut milk
Definition of tissue culture
Tissue culture is a technique where parts of a selected plant are aseptically transferred to an appropriate culture medium containing the required growth factors, leading to the formation of an entire plant.
Components of the tissue culture media
The medium used for tissue culture usually contains macronutrients, micronutrients, nitrogen source, carbon source (sucrose), solidifying agents (agar) and plant growth regulators.
Agar agar
Agar agar is a polysaccharide obtained from seaweed that is used as a solidifying agent for semi-solid or solid nutrient media.
Micronutrients
Micronutrients like iron, copper, molybdenum are added in specific concentration to the tissue culture medium.
Glucose
Glucose can also act as a carbon source in the tissue culture medium, but sucrose is preferred.
Coconut milk
Coconut milk contains an abundant amount of zeatin which is a naturally occurring cytokinin. Cytokinins are hormones that stimulate cell proliferation of the roots and shoots of plants. Thus, the use of coconut milk, which is the liquid endosperm of the coconut, serves as a good source of phytohormones that are essential for the formation of plantlets in laboratory conditions.
Definition of tissue culture
Tissue culture is a technique where parts of a selected plant are aseptically transferred to an appropriate culture medium containing the required growth factors, leading to the formation of an entire plant.
Components of the tissue culture media
The medium used for tissue culture usually contains macronutrients, micronutrients, nitrogen source, carbon source (sucrose), solidifying agents (agar) and plant growth regulators.
Agar agar
Agar agar is a polysaccharide obtained from seaweed that is used as a solidifying agent for semi-solid or solid nutrient media.
Micronutrients
Micronutrients like iron, copper, molybdenum are added in specific concentration to the tissue culture medium.
Glucose
Glucose can also act as a carbon source in the tissue culture medium, but sucrose is preferred.
Coconut milk
Coconut milk contains an abundant amount of zeatin which is a naturally occurring cytokinin. Cytokinins are hormones that stimulate cell proliferation of the roots and shoots of plants. Thus, the use of coconut milk, which is the liquid endosperm of the coconut, serves as a good source of phytohormones that are essential for the formation of plantlets in laboratory conditions.
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