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Question

In-vitro template independent RNA synthesis is a feature of

A
RNA polymerase
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B
Reverse ranscriptase
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C
Ochcoa enzyme
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D
DNA polymerase
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Solution

The correct option is C Ochcoa enzyme
The RNA polymerase is an enzyme that can synthesize the RNA in-vitro but it needs DNA template to do that. An enzyme called polynucleotide phosphorylase, also called Ochoa enzyme can synthesize template-independent RNA in-vitro. This enzyme uses ribonucleoside diphosphates instead of ribonucleoside triphosphates used by RNA polymerase. The same enzyme breaks down RNA in-vivo.
A. RNA polymerase needs DNA template.
B. Reverse transcriptase synthesizes DNA from the RNA template.
C. Ochoa enzyme can make template-independent RNA in-vitro.
D. DNA polymerase makes DNA from DNA template.
Hence, the correct answer is 'Ochoa enzyme'

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