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Question

In which of the following biochemical experiments UV spectrophotometer is used for estimation?

A
DNA and protein isolation
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B
Enzyme kinetics
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C
RNA isolation
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D
All of the above
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Solution

The correct option is D All of the above
DNA and RNA absorb UV at 260 nm can also be quantified by measuring the UV-induced emission. UV spectroscopy is also used to determine the kinetics or rate constant of a chemical reaction. The reaction occurring in solution gives color or brightness shifts from reactants to products that can be detected by UV spectrophotometry. Thus, the correct answer is option D.

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