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Question
In which of the following method, foreign DNA is injected into a cell with calcium ions?
In which of the following method, foreign DNA is injected into a cell with calcium ions?
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Solution
The correct option is A Calcium phosphate precipitation
The desired gene of the recombinant DNA can be incorporated into the host cell by different methods. There is no involvement of the vector in the gene transfer method, which is known as the direct gene transfer. Different chemicals like PEG and calcium phosphate are used to make the cells competent which can take up the recombinant DNA. The calcium ions are used which allow creating pores in the cell wall. This makes the cell wall permeable and the foreign gene can be injected into the cell.Thus, the correct answer is option A.
The desired gene of the recombinant DNA can be incorporated into the host cell by different methods. There is no involvement of the vector in the gene transfer method, which is known as the direct gene transfer. Different chemicals like PEG and calcium phosphate are used to make the cells competent which can take up the recombinant DNA. The calcium ions are used which allow creating pores in the cell wall. This makes the cell wall permeable and the foreign gene can be injected into the cell.
Thus, the correct answer is option A.
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