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Question

In which of the following phase the measured optical density is highest?
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A
Lag phase
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B
Log phase
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C
Stationary phase
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D
All of the above
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Solution

The correct option is B Log phase
In the log phase, each bacteria undergoes reproduction by the division to produce daughter cells, and the progeny cells are also committed to doubling as long as conditions remain favorable. This constant doubling time produces an exponentially increasing population count for the bacteria giving highest optical density in UV spectrophotometry. This stage is known as log phase or exponential phase because plotting the log of the cell number against time gives a straight line. Thu,s the correct answer is option B.

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