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Question

In which of the following reaction of glycolysis, a molecule of water is removed from the substrate?

A
Frucoste-6-phosphate → Fructose 1, 6-phosphate
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B
3-phosphate glyceraldehyde → 1, 3-biphosphoglyceric acid
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C
PEP → Pyruvic acid
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D
2-phosphoglycerate → PEP
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Solution

The correct option is D 2-phosphoglycerate → PEP
During glycolysis, dehydration occurs in the presence of enzyme enolase and cofactor Mg2+ . 2-phosphoglycerate loses a molecule of water and is changed into phosphoenol pyruvate (PEP).

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