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Question

In which of the following reactions of glycolysis does oxidation take place?

A
Glucose-6-phosphate to fructose-6-phosphate
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B
Glyceraldehyde-3-phosphate to 1,3-diphosphoglycerate
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C
1,3-Diphosphoglycerate to 3-phosphoglycerate
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D
2-Diphosphoglycerate to phosphoglycerate
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Solution

The correct option is B Glyceraldehyde-3-phosphate to 1,3-diphosphoglycerate
Glyceraldehyde-3-phosphate is oxidized and phosphorylated to form 1,3-diphosphoglycerate, and this process results in the reduction of NAD+:

Glyceraldehyde-3-phosphate + NAD+ + Pi 1,3-Diphosphoglycerate + NADH

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