The correct option is D Pisces only
Pisces have two chambered heart one atria and one ventricle. They have a closed loop circulatory system. The deoxygenated blood enters the atria through sinus venosus. The atrium serves as a one-way antechamber, sends blood to the third part, ventricle. The ventricle is another thick-walled, muscular chamber and it pumps the blood, first to the fourth part, bulbus arteriosus, a large tube, and then out of the heart. The bulbus arteriosus connects to the aorta, through which blood flows to the gills for oxygenation.
So, the correct answer is option D.