Question

In which step does the gene of interest get incorporated into the plant genome?

• A. Growing the discs with shoot inducing medium
• B. Growing the leaf discs with agrobacterium
• C. Cutting the leaf discs
• D. Growing the discs with root inducing medium

Answer 1

The correct option is B Growing the leaf discs with agrobacterium

In the given process, transformation occurs when the cells are infected with agrobacterium containing a tamed Ti plasmid with the gene of interest inserted in the place of the tumor inducing gene. The desired gene gets integrated with plant genome. Cells are then allowed to redifferentiate into roots and shoots by growing them in appropriate media containing growth hormones for shoot and root development forming the transgenic plant.