Question

Inner & outer membrane in mitochondria separated by reaction of detergents & ultra vibrations why?

Answer 1

The inner and outer mitochondrial membranes are separated by an intermembrane space. Inner and outer membranes in mitochondria are separated by controlled osmotic lysis, ultra vibration, non-ionic detergent followed by differential centrifugation. Digitonin is a common non-ionic detergent used for separation of inner and outer mitochondrial membrane. The inner membrane consists of cardiolipin, which is resistant to digitonin. Whereas it can lyse the outer membrane.