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Reproduction in Bacteria

Insertional i...

Question

Insertional inactivation is practiced in genetic engineering for

A

Multiplying desired DNA fragments.

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B

Ligating desired DNA segment to the vector

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C

The selection of virulent bacterial colonies

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D

The selection of recombinant bacterial colonies

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Solution

The correct option is D
The selection of recombinant bacterial colonies

Insertional inactivation is a method in genetic engineering for selection of recombinants. In this method, a gene is inserted into another gene to inactivate it. During transformation, this technique helps in selecting the transformed cells.

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Similar questions

Q. How many of the following statement(s) regarding insertional inactivation of lacZ gene are incorrect?

1. lacZ gene remains active in non-recombinant transformed bacteria.
2. X-gal gets hydrolysed and produces a blue product in non-recombinant transformed bacteria.
3. Bacterial colonies which are transformed with recombinant DNA appear white.
4. 𝛃-Galactosidase is produced in recombinant bacteria.
5. Bacterial colonies which are transformed with recombinant DNA appear blue.

Q. Bacterial colonies that have a foreign DNA fragment inserted in to the plasmid will appear white because:

Q. Write any four ways used to introduce a desired DNA segment into a bacterial cell in recombinant technology experiments.

Q. Read the given statements and select the correct option.
Statement-1: In insertional inactivation, blue colour produced by bacterial colonies indicates that the plasmid does not have an insert into the bacterial genome.
Statement-2: Presence of insert results into insertional inactivation of beta- galactosidase enzyme and the colonies do not produce any colour.

Q. After insertion of DNA segment within the sequence of Z-gene of bacteria, they are grown on chromogenic substrate. After the growth of bacterial colonies, they are identified as
i) Recombinants if colonies are blue- coloured.
ii) Recombinants if colonies are white coloured.
iii) Non-recombinants if colonies are blue- coloured.
iv) Non-recombinants if colonies are white coloured.

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