Question

Intact chloroplasts of green cells can be isolated by

• A. Alcohol
• B. Acetone
• C. Carbon disulphide
• D. Sugar solution

Answer 1

Answer: (D)

Sugar solution

• The chloroplast is, in general, an extremely fragile structure. Thus, in order to avoid large-scale rupture of the two envelope membranes during chloroplast isolation, sugar solution can be used to avoid osmotic shock and thus, disruption of the organelles.
  
• Intact chloroplasts of green cells are the best source for studying the processes like carbon assimilation, electron flow and phosphorylation.
  
• So the correct option is 'Sugar solution'.