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Question

Intact chloroplasts of green cells can be isolated with the help of

A
Acetone
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B
Alcohol
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C
Carbon disulphide
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D
0.5 M solution of sucrose
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Solution

The correct option is D 0.5 M solution of sucrose
Chloroplast can be isolated in tris-HCl buffer made 0.5M with respect to the sucrose. Here, the extracts of the chloroplast extracted with the sucrose solution had considerable enzymatic activity and did not inhibit this activity.
Thus, the correct answer is option (D), '0.5 M solution of sucrose'.

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