Question

Internal phosphodiester bond in a nucleic acid molecule is hydrolysed by _______________.

• A. Exonuclease
• B. Endonuclease
• C. DNA polymerase
• D. Deoxyribonucleic acid

Answer 1

The correct option is B Endonuclease

• In DNA and RNA, the phosphodiester bond connects a 3'-carbon to a 5'-carbon which is hydrolyzed by the endonuclease restriction enzyme.
• Endonuclease makes cuts within the DNA strands while exonuclease removes nucleotides from the end part of the DNA.
• DNA polymerase enzymes synthesize DNA molecules from deoxyribonucleotides.

Hence, the correct answer is option B, i.e., Endonuclease.