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Question 32

Is extragenomic DNA present in prokaryotes and eukaryotes? If yes, indicate their location in both the types of organisms.

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Solution

yes, extragenomic DNA is present in both prokaryotes and eukaryotes. In case of eukaryotes, extragenomic DNA is present in two organelles, e.g., plastids and mitochondria.

Extrachoromosomal DNA in Prokaryotes In case of prokaryotic cells, the extragenomic DNA is present in the form of plasmids. The plasmids are circular DNA molecules, which confer certain unique phenotypic characters to the bacteria.

One such character is antibiotic resistance to bacteria. The plasmid DNA is also used to monitor bacterial transformation with foreign DNA. Bacteria transformation is the method of sexual reproduction in bacteria.

Extrachoromosomal DNA in Eukaryotes

Mitochondrial DNA (mtDNA) is usually circular. Mitochondrial DNA spans about 16,500 DNA building blocks (base pairs) representing a small fraction of the total DNA in cells.

mtDNA contains 37 genes, all of which are essential for normal mitochondrial functions.

Thirteen of these genes provides instruction for making enzymes involved in oxidative phosporylation.

Mitochondrial genes are among the estimated 20000-25000 total genes in the human genome.

Plastids These are small bodies found free in most plant cells and are of three types leucoplast, chromoplast and chloroplast. These are double membrane bound structures for trapping radiation and storage purpose.

It contains small, double stranded circular DNA molecules and ribosomes essential in synthesising certain proteins. As they both can synthesis certain proteins. As they both can synthesis proteins and replicate on their own they are known as semi-autonomous organelles.


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