Question

Is pBR322 artificial?

Answer 1

pBR322:

1. pBR322 is a vector plasmid created from bacteria by the scientists Bolivar and Rodriguez.
2. It is an artificial cloning vector used to clone a specific gene of interest inside E.coli.
3. It is one of the most widely used E.coli vectors.
4. BR in the pBR322 is an acronym for the scientist's Bolivar and Rodriguez.
5. It has the restriction enzyme (enzymes that cut the DNA at specific sites) sites for Pst1, EcoR1, pVU2, and BamH1.
6. It has different regions such as the origin of replication, sites for restriction enzymes, resistance from antibiotic tetracycline and ampicillin resistance, etc.