These plants use the Calvin pathway for the dark reactions (light-independent) to synthesize food using photosynthesis.
They synthesize a 3-carbon product, i.e., phosphoglyceric acid at the end of the reaction.
These plants can survive in temperate conditions and are found in abundance.
C4 plants:
C4 plants use the hatch and slack pathway for the dark reactions of photosynthesis.
C4 plants produce a 4-carbon carbohydrate at the end known as oxaloacetic acid.
C4 plants are found in tropical conditions in abundance.
PEP carboxylase:
PEP carboxylase is the first enzyme that helps in carbon fixation.
PEP carboxylase is present in the C3 (follow Calvin cycle in which initial assimilation of CO2 produces 3 carbon molecule) plants where it produces 3-phosphoglycerate to replenish citric cycle intermediates and is also involved in the functioning of stomata.
Whereas in C4(Initial assimilation of CO2 produces 4 carbon molecule) plants it is used for the conversion of initial CO2 to oxaloacetate and then to phosphoenol pyruvate (PEP).
This PEP acts as a primary CO2 acceptor which is later converted to oxaloacetic acid (OAA) by the enzymatic action of PEP carboxylase.
PEP carboxylase also helps in pH regulation and stomatal opening.