Question

Is there any relationship between dedifferentiation and the higher degree of success achieved in plant tissue culture experiments?

Answer 1

Dedifferentiation:

Dedifferentiation is a phenomenon by which a differentiated plant cell regains the capacity of cell division and becomes an undifferentiated meristematic cell again. Example: differentiated parenchyma cells dedifferentiate to produce cork cambium of a plant.

Dedifferentiation is responsible for successful plant tissue culture:

Dedifferentiation is responsible for successful plant tissue culture. The differentiated cells of the explant dedifferentiate and become meristematic under the influence of plant growth regulators in the tissue culture medium. These cells divide and form a mass of undifferentiated cells known as callus.

When subjected to appropriate concentrations of specific growth hormones, the cells of the callus undergo differentiation, form organs and ultimately develop into a plantlet that can grow into a whole plant when transferred to a field.