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Cell Wall

Isolation of ...

Question

Isolation of DNA from a fungal cell involves the use of enzyme

A

Chitinase

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B

Lysozyme

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C

Eco RI

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D

Hind II

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Solution

The correct option is A Chitinase
Chitinase is an enzyme that degrades chitin, which is a chief constituent of fungal cell walls.

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Q. Recombinant DNA technology involves several steps in which initial step is of isolation of the DNA. Which enzymes are used in the process for the break down of fungal cell, plant cell and bacterial cell respectively?

Q. Name the enzymes that are used for the isolation of DNA from bacterial and fungal cells for recombination DNA technology.

Q. Assertion :Use of chitinase enzyme, is necessary for isolation of DNA from yeast cells but not in case of Spirogyra. Reason: Fungal cell wall is made up of fungal cellulose or chitin.

Q. Give reasons for the following:
For isolating the genetic material from a fungal cell chitinase enzyme is used.

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