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Question

Isopycnic centrifugation is also known as

A
Density gradient centrifugation
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B
Zonal centrifugation
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C
Differential centrifugation
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D
All of the above
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Solution

The correct option is A Density gradient centrifugation
In density gradient centrifugation also called isopycnic separation, or equilibrium separation, particles are separated only on the basis of their density. Particle size affects the rate at which particles move until their density is the same as the surrounding gradient medium. The density of the gradient medium should be greater than the density of the particles to be separated. In this method, the particles will never sediment to the bottom of the tube. Thus the correct answer is 'Density gradient centrifugation'.
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