Question

It shows correct chronological order of the events occurring during callus culture

• A. Callus -> Cell division -> Explant -> Addition of cytokinin -> Acqire meristematic property
• B. Explant -> Callus Cell division -> Addition of cytokinin -> Cells acquire meristematic property
• C. Explant -> Cell division -> Callus -> Addition of cytokinin -> Cells acquire meristematic property
• D. Callus -> Explant -> Cell division -> Addition of cytokinin -> Cells acquire meristematic property

Answer 1

Answer: (C)

Explant -> Cell division -> Callus -> Addition of cytokinin -> Cells acquire meristematic property

Callus cell culture is formation of new plants from few cells obtained from a desirable plant.

A small piece of the plant is taken and placed in a nutrient medium.

This small piece is called explant, the explant then undergoes cell division to form an undifferentiated mass of cells called the callus

The callus is then added with cytokinin to acquire meristematic activity

So, the solution is C 'Explant -> Cell division -> Callus -> Addition of
cytokinin -> Cells acquire meristematic property'