Question

Large increase in absorption of UV- radiation of 260 nm by melted DNA duplex is called

• A. Gaidukov phenomenon
• B. Position effect
• C. DNA compaction
• D. Hyperchromic effect

Answer 1

The correct option is D Hyperchromic effect

• The increase in the absorbance of a material is known as hyperchromicity and the effect is known as the hyperchromic effect.
• The DNA molecule gets denatured by the hyperchromicity.
• The absorption of UV radiation by DNA molecule increases when the two single DNA strands get separated by heat or by a heat denaturant or by changing the pH level.
• So, the correct answer is 'Hyperchromic effect'.