The correct option is
D At the middle of the cycle
Menstrual cycle starts with the menstrual flow (menses), this is followed by follicular phase. During this phase secretion of gonadotropins (LH and FSH) and estrogens. In the middle of the cycle, rapid secretions of LH and FSH increases leading to LH surge and hence ovulation. Ovum lasts for 24 hours only. It is followed by the luteal phase during which the remaining parts of Graafian follicle changes into corpus luteum.
So, the correct option is 'At the middle of the cycle'.