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PCR in Diagnosis

Ligase is an ...

Question

Ligase is an enzyme required for

A

Breaking of DNA fragments

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B

Joining DNA fragments

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C

Renaturation of DNA

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D

Proof reading

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Solution

The correct option is B Joining DNA fragments
Ligase enzyme is that ligates (joins) the fragments of DNA by forming the phosphodiester bond. Ligase is very useful during DNA replication due to the formation of Okazaki fragments, which needs ligation after the process of replication is over.

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