Question

Liposome are prepared for liposome-mediated gene transfer by a process known as

• A. Spectrophotometer
• B. Centrifugation
• C. Reverse-phase evaporation
• D. None of the above

Answer 1

The correct option is C Reverse-phase evaporation

Liposome are prepared for liposome-mediated gene transfer by a process known as reverse phase evaporation. It is based on the creation of inverted micelles. These inverted micelles are shaped upon sonication of a mixture of a buffered aqueous phase, which contains the water-soluble molecules to be encapsulated into the liposomes and an organic phase in which the amphiphilic molecules are solubilized. The slow elimination of the organic solvent leads to the conversion of these inverted micelles into viscous state and gel form. At a critical point in this process, the gel state collapses, and some of the inverted micelles were disturbed. The excess of phospholipids in the environment donates to the formation of a complete bilayer around the residual micelles, which results in the creation of liposomes.

So, the correct answer is option C.