Question

List different types of chromatography.

Answer 1

Chromatography:

• This is one of the latest techniques to separate the components of a mixture when all the components are very similar in their properties.
• Chromatography is a technique that is used to separate a mixture of chemicals in a liquid or gaseous form based on the differences in absorbency (the quality of being able to adsorb something easily).
• The technique of chromatography is based on the principle of selective adsorption (surface adsorption) of different components on the surface of a suitable adsorbent.
• Chromatography consists of a mobile phase and a stationary phase.
• The mobile phase is a fluid or a solvent that carries the components of the mixture to be separated through the fixed column.
• The stationary phase is the column that stays fixed and the solvent moves through it.
• For example, in paper chromatography, the paper is the stationary phase and the solvent might be water or any other solvent that helps to separate the constituents of the mixture.
• Various chromatography methods have been developed to that end.
• Some of them include column chromatography, thin-layer chromatography (TLC), paper chromatography, gas chromatography, ion exchange chromatography, gel permeation chromatography, high-pressure liquid chromatography, and affinity chromatography.